Practical Manual of Assisted Reproductive Technology Lab

This present practical manual of ART is an outcome of my personal and professional clinical ART experience carried out during the period 2009 – 2022 with various IVF centers in north India. It was my privilege to observe numerous clinical laboratories works under the supervision of senior clinical embryologists, as well as hands-on IVF andrology laboratory work independently. As a result, I would like to share my experience in the form of a textbook entitled “Practical Manual of Assisted Reproductive Technology (ART) Lab”.
In this practical manual have 20 practical’s with a well-explained working protocol as per WHO 2021. 1st practical is semen analysis (SA) and it is explained as the Objective, Principle, and indication of SA and solution of abnormal SA report, material requirements, method of SA, and reporting format with a practice sheet. Similarly, all the practical’s have these types of explanations. 2nd practical is sperm morphology (SM). 3rd practical is the sperm survival test (SST). 4th practical is the sperm vitality test (SVT). 4.1 practical is the hypo-osmotic test (HOST). 4.2 practical is the eosin-nigrosin test. 4.3 practical is a motility test for necrozoospermia. 5th practical is the sperm DNA fragmentation index (SDFI). 6th test is a semen preparation for IUI & IVF / ICSI or ART procedure. 7th practical is the sperm function test (SFT). The 8th practical is a post-coital test (PCT). 9th practical is the sperm-cervical mucus contact (SCMC). 10th practical is a semen fructose test. 11th practical is the retrograde semen ejaculation test. 12th practical is the Processing of TESA sample. 13th is the general awareness about culture media, dish preparation and oocyte preparation for IVF/ICSI. 14th practical is the oocyte & embryo cryopreservation and thawing. 14.1 practical is the sperm / semen cryopreservation. 14.2 practical is the sperm / semen thawing. 15th practical is the last practical of this manual which is catheter flushing, embryo loading and ET.
The purpose of this practical manual is to provide basic information about how to perform the andrology and ART lab tests in a precise manner. This manual is intended for Beginner, B.Sc., M.Sc., and Ph.D. scholars in clinical embryology, as well as ART practitioners. A practical manual providing principal-based approaches to aid in the completion of ART laboratory test in a systematic manner is presented in this book.